Specific antibodies to Besnoitiabesnoiti precipitated from serum of cattle by live parasites and by soluble antigen

Abstract Specific precipitable antibodies of both IgG and IgM classes were detected in sera of cattle naturally infected with B. besnoiti . The amount of specific antibodies of the IgG class precipitated by soluble antigen was in the range of 17–50 μg/ml serum while that of the IgM class ranged between 5 and 24 μg/ml serum. Specific antibodies precipitated by live B. besnoiti parasites were in amounts of 10 to 22 μg/ml serum for IgG and 4 to 26 μg/ml serum for IgM. Different ratios of IgG/IgM were obtained by the two methods of precipitation. This might indicate that antibodies to B. besnoiti of the IgM class can be precipitated and detected in sera of naturally infected cattle in similar amounts either by live parasites or by solubilized antigen, whereas antibodies of the IgG class can be preferentially detected when solubilized antigen is used for precipitation.