Infection ofMacrophages withLegionella pneumophila Induces Phosphorylation ofa 76-Kilodalton Protein

or phorbol myristate acetate. However, phosphorylation didoccur inmacrophages infected witha virulent strain ofL. pneumophila andtreated witheither erythromycin toinhibit growth orwithcytochalasin D toinhibit uptake ofL.pneumophila bymacrophages. Theseresults support theviewthatphosphorylation ofthis protein occurs during theearlyphases ofinteraction between L.pneumophila andmacrophages. Theroleofthisspecific protein intherecognition, intracellular uptake, andgrowthofL.pneumophila inpermissive macrophages remains tobeclarified.