[Influence of stir-baked with sand on active ingredients, diarrhea and hepatoprotection of Herpetospermum caudigerum].

To study the influence of stir-baked with sand on active ingredients,diarrhea and hepatoprotection of Herpetospermum caudigerum,the contents of herperione and herpetin in H. caudigerum before and after stir-baking with sand were analyzed by HPLC.The effect of stir-baked with sand on diarrhea of H. caudigerum TL was evaluated using the mean stool rate( MSR) and mean diarrheal index( MDI) and the influence of stir-baked with sand on hepatoprotective effect of H. caudigerum TL was examined using a mouse model of CCl4-induced liver injury based on the analysis of serum ALT and AST activities. The results of HPLC analysis showed the content of herperione in H. caudigerum after stir-baking with sand decreased by 40. 9%( P 0. 01) and the content of herpetin had no change. Pharmacodynamic results showed that the MSR and MDI of high-dose and middle-dose group of H. caudigerum TL after stirbaking with sand were significantly lower than that of high-dose and middle-dose group of H. caudigerum TL without stir-baking with sand; The high-dose and middle-dose of H. caudigerum TL with / without stir-baking with sand significantly alleviated liver injury as indicated by the decreased levels of serum ALT and AST,but the ALT and AST levels of high-dose and middle-dose group of H. caudigerum TL after stir-baking with sand were higher than that of H. caudigerum TL without stir-baking with sand. The results revealed that the stir-baking with sand could effectively relieve diarrhea effect of H. caudigerum TL,while it also reduces the hepatoprotection of H. caudigerum TL.