Study on the comparability of measured HBDH results with different biochemical detecton systems

Objective To explore the comparability of α-hydroxybutyrate dehydrogenase(HBDH) results of different biochemical detection systems.Method Refering to EP9-A file of NCCLS,different biochemical detection systems,detecting system 1-2(laboratory method) and detection system 3(target system :comparison method,which includes Hitachi 7170 biochemical analyzer,reagent of Roche,c.f.a.s.calibrator and controller of Roche approved by ISO/IEC 17025),were used to 2 serum enzyme items such as HBDH to obtain the correlation coefficient and linear equation respectively.Then the coefficient and equation were used to evaluate the system bias between laboratory method and comparison method.The comparability of different investigate systems was judged according to the half of CLIA'88 standard.Results The system bias of HBDH could be accepted between several investigate systems and target system.Conclusion The comparability of HBDH results in 3 kinds of detection system accorded to the evaluation of clinical acceptability.If there are more than two systems for the same test,it is necessary to do method comparison and Bias estimation in order to einsure the comparability.