The Determination of PAHs in Fish by GC-QqQ-MS/MS

A new method was developed to determine the contents of PAHs in fish by gas chromatography–triple quadrupole mass spectrometry (GC–QqQ-MS/MS) with SRM mode. In this method, ultrasonic extraction with hexane/dichloromethane (1:1v/v) and Florisil column cleaning with hexane/acetone (4:1v/v) was used as the sample pretreatment. Recoveries of the 16 PAHs for spiked samples were between 68.5% and 106.3%, the relative standard deviations (RSD) were between 0.1% and 17.8%. The limits of detection (LODs) ranged from 0.024–0.06 μg/kg and the limits of quantitation (LOQs) ranged from 0.08–0.2 μg/kg. The influence of different elution solvent to recovery and the matrix effect were also discussed.