Effect of deproteinizing agents on biochemical variability of total protein in Cotugnia digonopora

Biochemical profiles are subject of variation. Physiological state of the host exhibits profound influence on biochemical individuality of parasite. Methods for various biochemical assays which have been described in many journals are mainly pertaining to mammalian model. The same may not be good for other animal model particularly for helminth parasites. In view of above mentioned facts there is a need to evaluate various existing methods and also to modify the method suitably, to cope up with complexities of biochemical profiles of helminth tissue. The present investigation is planed to evaluate a suitable method related to estimate the total protein quantitatively in the cestode Cotugnia digonopora . Therefore in present investigation is aimed to know the biochemical variability of certain selected biochemical assay related to total protein of cestode Cotugnia digonopora occurred in domestic fowl Gallus gallus domesticus in Maharashtra, India. In the light of present findings it would seem to logical to recommend that a Biuret assay for the cestode parasites, when compared to Folin-Phenol method.