Modification of PMR Spectroscopy Technique for Determination of the Molar Substitution in Hydroxyethyl Starch

The PMR spectroscopy technique for determining the molar substitution (MS) in hydroxyethyl starch (HES) was modified. It was shown that allowance for integrated intensities of resonances for anomeric H(1) protons of terminal and branching substituted and unsubstituted α-D-glucopyranose residues in HES decreased the calculated MS values and eliminated differences in the MS values obtained by PMR spectroscopy and gas chromatography.