Seroprevalence of Campylobacter spp. Using Enzyme-Linked Immunosorbent Assay in Humans and Chickens from Selected Areas of Metro Manila and Laguna, Philippines

An enzyme-linked immunosorbent assay (ELISA) was adopted to determine the occurrence of antibodies for Campylobacter jejuni/coli and C. jejuni O:19 in 128 human and 128 chicken serum samples. Seroprevalence to C. jejuni/coli among humans tested was found very high at 96.1% (123/128), compared to C. jejuni O:19 at 0.6% (2/128). This is the first report on C. jejuni/coli seroprevalence in the country. Moreover, it is also the first report for serodiagnosisof C. jejuni O:19 in the Philippines. The prevalence rates for antibodies to C. jejuni/coli and C. jejuni O:19 among different age groups and between sexes were not significantly different(P Keywords: antibodies, Campylobacter, ELISA, seroprevalence