Method for detecting hatchability of insect eggs by using spermatin gene
2013
The invention discloses a method for detecting the hatchability of insect eggs by using a spermatin gene. The invention provides an application of a substance for detecting the expression level of a spermatin CSSFP066-coding gene in insect populations fed with different substances in detection of the hatchability of the eggs of the insect populations fed with different substances; and the amino acid sequence of a spermatin CSSFP066 is sequence 2 in a sequence table. According to the method disclosed by the invention, experiments prove that the detection method provided by the invention can be used for detecting the hatchability of the insect eggs, in particular to the hatchability of the eggs of arma chinensis with different nutrient sources; and the detection of the hatchability of the insect eggs by the detection method disclosed by the invention only requires 2-3 days. However, the use of a traditional biological method requires about 30-60 days. The detection method disclosed by the invention has the advantages of wide range of involved material sources, easiness in purchase, simplicity in operation, and time-saving and labor-saving properties, and is suitable for popularization and application in commodity inspection and detection of insects.
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