High cell density fed-batch culture of E.coli expressing rhVEGF121
2005
Objective To investigate the optimal high cell density fermentation conditions of recombinant E.coli BL21/pET- 24a/hVEGF121 expressing recombinant human vascular endothelial growth factor (rhVEGF121). Methods The effects of the composition of the fermentation medium, induction time and fed-batch carbon sources on the expression level of rhVEGF121 and cell output were analyzed. Results and Conclusion When cultured in modified M9 medium and induced for 4 h in the presence of 0.5 mmol/L IPTG at 37 ℃ with glycerol as the carbon sources by continuous fed-batch mode, the recombinant E.coli expressed rhVEGF121 at the level up to 23% of the total proteins and the yield reached 68 g/L. The optimized fermentation condition for recombinant E.coli enables high expression level of rhVEGF121.
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
0
References
0
Citations
NaN
KQI