31P NMR phospholipid profile study of seven sea anemone species

Abstract 1. 1. Phospholipids were extracted from 7 species of sea anemones collected from the Caribbean coast of Puerto Rico and analyzed by 31 P NMR. 2. 2. Fifteen resonances were detected: phospholipids, No. 1 (AEP No. 1, chemical shift, 21.19 °), No. 2 (AEP No. 2, 20.38 δ), and no. 3 (AEP No. 3, 21.01 δ); PG (0.50 δ); PA (0.25 δ); CL (0.18 δ); PE (0.03 δ); PS (−0.05 δ); PI (−0.32 δ); PE plas (0.07 δ); PC plas (−0.78 δ); LPC plas (−0.20 δ); LPC (−0.28 δ); and an unknown phospholipid phosphate resonance at −0.88. 3. 3. High concentrations of phosphono derivatives (23.26 ± 1.24 mole/%)PE plas (23.60 ± 0.84), and PC plas (19.74 ± 2.24) were found. 4. 4. In the concentration range between 5 and 10%, PC and PS were found. 5. 5. PG, PE, PI, LPC plas, and LPC were found in a concentration range between 1 and 5%. 6. 6. The detected PA was less than 1%. LPH was not detected. 7. 7. Phosphonolipid No. 1 correlated negatively vs PC plas, as well as vs AEP No. 3, and PE. 8. 8. Positive correlation was found between the total choline-containing phospholipids and PC plas. 9. 9. The comparison of the phospholipid concentrations among these species anticipates the possibility of studying anemone classification based upon anemone phospholipid types and relative concentrations. 10. 10. These results indicate that the anemone cell membrane phospholipid structure follows certain specific intrinsic patterns depending on species.