Rupture of Lipid Membranes Induced by Amphiphilic Janus Nanoparticles

The surface coatings of nanoparticles determine their interaction with biomembranes, but studies have been limited almost exclusively to nanoparticles with a uniform surface chemistry. Although nanoparticles are increasingly made with complex surface chemistries to achieve multifunctionalities, our understanding of how a heterogeneous surface coating affects particle–biomembrane interaction has been lagging far behind. Here we report an investigation of this question in an experimental system consisting of amphiphilic “two-faced” Janus nanoparticles and supported lipid membranes. We show that amphiphilic Janus nanoparticles at picomolar concentrations induce defects in zwitterionic lipid bilayers. In addition to revealing the various effects of hydrophobicity and charge in particle–bilayer interactions, we demonstrate that the Janus geometry—the spatial segregation of hydrophobicity and charges on particle surface—causes nanoparticles to bind more strongly to bilayers and induce defects more effectively t...