Hapten Synthesis and Monoclonal Antibody-Based Immunoassay Development for the Analysis of Thidiazuron

Thidiazuron (TDZ) is a synthetic plant growth regulator, which is widely used in agriculture and tissue culture. A sensitive and specific monoclonal antibody (mAb3D6F5B2) was obtained. An indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed with mAb3D6F5B2. The established icELISA possessed a 50 % inhibition concentration (IC50) of 0.61 ng/mL and a quantitative range of 0.15–2.49 ng/mL. The recoveries of TDZ fortified in water and soil samples were 76–126 %. The residual level of TDZ in soil samples detected by icELISA was confirmed with high performance liquid chromatography-mass spectrometry (LC/MS). The correlation coefficient (R 2) between the two assays was 0.9898, demonstrating this icELISA is suitable to detect TDZ residues in environmental and agricultural samples.