A 500-kb physical map and contig from the Harvey ras-1 gene to the 11p telomere.

Abstract A contiguous physical map was constructed from the Harvey ras-1 ( HRAS1 ) gene to the 11p telomere. The contig spans approximately 500 kb and is minimally composed of a telomere-containing YAC and P1 and cosmid clones. Included in the contig are 11 sequence-tagged sites derived from P1 and cosmid ends. Three genes were placed on the contig in the following order: telomere–ribonuclease/angiogenin inhibitor ( RNH )–Harvey ras-1 ( HRAS1 )–HRAS1-related complex ( HRC ). Two novel tetranucleotide repeats (heterozygosity of 66 and 68%) and a complex CA repeat (heterozygosity of 78%) were isolated and characterized.