Activity of Torpedo Californica Acetylcholinesterase in the Crystalline State

Numerous studies have demonstrated that enzymes display catalytic activity in the crystalline state [1]. Factors which might affect activity of a crystalline enzyme include: i. Ability of substrates to reach the active site and of products to exit ii. Chemical composition of the crystallisation mother liquor (ML) iii. Conformational freedom of residues involved in substrate binding and hydrolysis