Coated-bead assay for microbial cell-associated and extracellular protease activity

: An assay utilizing latex beads coated with substrates of 125I-labelled connective tissue proteins is described as an alternative method for quantitating cell-associated and extracellular proteolytic activity of micro-organisms. Proteolytic activity is a function of the amount of 125I-labelled peptides released into the incubation mixture.