Characteristics of Larval Breeding Sites and Insecticide Resistance in the Anopheles gambiae Complex in Mpumalanga, South Africa

The larval breeding sites of anopheline mosquitoes in eastern Mpumalanga Province, South Africa, were investigated. Using abiotic parameter data collected in the field, environmental conditions that correlate with larval community composition and productivity are reported. Twelve anopheline species were identified, with the malaria vector Anopheles arabiensis Patton (Diptera: Culicidae) ranking as the second most abundant. There was a significant difference in the mean abundance of each species of anopheline from each of the geographically separate breeding sites (Kruskal-WallisANOVA, P<0.05, d.f.= 6,H 2 = 17.09). Anopheles merus Donitz showed a significant positive correlation with salinity (Pearson's product moment, r = 0.971, P < 0.05). Habitats such as rain puddles, hoof prints and residual pools left after rivers recede were important breeding sites and these were characteristically short-lived, and fully or partially sunlit. Anopheles gambiae complex members from the breeding sites surveyed during t...