SIMULTANEOUS HIGH - PER FORMANCE LIQUID CHROMATOGRAPHIC DETERMINATION OF IRBESARTAN AND HYDROCHLOROTHIAZIDE IN PHARMACEUTICAL DOSAGE FORM

A simple, rapid, accurate and precise method is developed for the quantitative simultaneous determination of Irbesartan and Hydrochlorothiazide in combined pharmaceutical dosage form. Separation is achieved with an Ace5 - C 18, ( 250 X 4.6)mm,5 μ analytical column using buffer - acetonitrile (65:35, v/v) of pH 5.5, adjusted with acetic acid as the mobile phase. The buffer used in mobile phase contains 50Mm ammonium acetate in double disti lled water. The instrumental setting is fl ow rate of 1 mL/ min, column temperature at 30 o C, and detector wavelength of 260 nm. The internal standard method is used for the quantitation of the ingredients of this combination. Methylparaben is used as an internal standard. The method is validated an d shown to Hydrochlorothiazide are 0.9998 and 0.9999 , respectively. The relative standard deviations for six replicate measurements in two sets of each drug in tablets are alway s less than 2%. be linear for Irbesartan and Hydrochlorothiazide . The correlati on coefficient for Irbesartan and Hydrochlorothiazide are 0.9998 and 0.9999 respectively. The relative standard deviation for six replicate measurements in two sets of each drugs in tablets are always less than 2%.