Preparation method of culture medium for mother seeds of lentinus edodes

The invention discloses a preparation method of a culture medium for mother seeds of lentinus edodes. The preparation method is characterized by comprising the following steps: S01, removing peels of potatoes, washing the peeled potatoes, cutting into slices, putting into an aluminum pot, adding water to boil on slow fire for 30-35 minutes, and filtering to obtain potato juice; S02, putting agar into water, soaking, adding the soaked agar into the potato juice, and heating further until the agar is melted completely, wherein the agar needs to be stirred into the potato juice with a glass rod in the process of heating; S03, adding glucose, potassium dihydrogen phosphate, sulfate magnesium and vitamin B1, and supplementing the solution with hot water until the volume of the solution reaches the set value; S04, testing the pH value of the solution and adjusting the pH value of the solution to 5.5-6; S05, filling the prepared culture medium into test tubes, and plugging the test tubes with tampons after finishing filling the prepared culture medium; S06, putting the test tubes into a high-pressure sterilization pot, and sterilizing; and S07, cooling, arranging the culture medium to be slant, cooling further, and solidifying to obtain the slant culture medium. The preparation process is simple and practical and has a good effect, and the culture medium prepared by adopting the preparation process can be used for collecting strains.