Two new lanostanoids from Ganoderma resinaceum

Two new lanostane triterpenoids, 3-epipachymic acid (3-acetoxy-16 alpha-hydroxy-24-methylene-5 alpha-lanost-8-en-21-oic acid, 1) and 3 alpha-(3-hydroxy-5-methoxy-3-methyl-1,5-dioxopentyloxy)-24-methylene-5 alpha-lanost-8-en-21-oic acid (2), together with a known compound, 3-oxo-5 alpha-lanosta-8,24-dien-21-oic acid (3), were isolated from the fruiting body of Ganoderma resinaceum. The structure elucidation was accomplished by spectroscopic methods, especially NMR experiments. Compound 2 showed significant cytotoxic activity with IC50 value of 2.5 mu g/ml in Hep-2 cell line.