Potential therapeutic use of RNA interference against COVID-19

Introduction: SARS-CoV-2 is the causal agent of COVID-19, a respiratory disease that has caused thousands of deaths globally for which there is no effective curative therapy Objective: To demonstrate the potential relevance of RNA interference (RNAi) technology as a therapeutic alternative in the treatment of COVID-19 Materials and methods: Specialized biomedical databases were searched looking for studies published until April 2020 The search was carried out using descriptors and Boolean operators Advanced search strategy was used for the selection of articles, taking into account the methodological quality and validity of the studies Results: Evidence of experimental application of RNAi technology against SARS-CoV was identified Several small interfering RNAs and small loop-structured RNAs oriented to the silencing of essential SARS-CoV genes including those encoding the S, RdRp, M, E, N, 3a/3b and 7a/7b proteins have been designed and evaluated The effectiveness of RNAi for silencing its target genes was proven Although most of these research studies have been conducted in in vitro systems, the therapeutic effectiveness of the intranasal administration of small RNA interference has also been proven in an in vivo SARS-CoV model Conclusions: RNAi technology has demonstrated to be a potential therapeutic strategy against SARS-CoV in cellular and animal models Given the similarities at the genomic level and in terms of the pathogenic process between SARS-CoV and SARS-CoV-2, this technology has a potential applicability for the treatment of COVID-19 © 2020 Universidad de Ciencias Medicas de La Hab All rights reserved