Saccharopolyspora spinosa recombinant strain with double bldD genes

2015 
The invention discloses a saccharopolyspora spinosa recombinant strain with double bldD genes. The provided saccharopolyspora spinosa recombinant strain with double bldD genes is prepared by integrating bldD gene into saccharopolyspora spinosa genome. The provided saccharopolyspora spinosa recombinant strain with double bldD genes is named as S.spinosa-BldD, and is preserved in the China Center for Type Culture Collection (CCTCC) in May 31st, 2015, and the preservation number is CCTCC No. M2015347. The PCR detection results show that bldD gene has been successfully integrated into a target bacterial strain chromosome. In the plate culture, people find that in BHI and TSB plates, the spores of recombinant strains are prominently inhibited. The results of shaking flask fermentation show that the spinosad yield of recombinant strain is increased by 1.35 times compared with that of a referential strain.
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