Effect of Tongru Sanpi decoction on expression of Bcl-2,PCNA of tissue of mammary glands in rats with hyperplasia of breast glands

[Objective] The aim of the present study was to detect correlation between gene expression of Bcl-2,PCNA and histology of hyperplasia of mammary glands and to explore the intervention therapy of Tongru Sanpitang on hyperplasia of mammary glands by studying the biological indicator of the cancer-changing tendency of hyperplasia of mammary gland.[Methods] Hyperplasia of mammary glands were induced in rats by intraperitonneal injection of Estradiol Benzoate and Progesterone.It was treated respectively by perfusion Tongru Sanpitang(high,middle and low doses) into stomach and in the control drug,Rupixiao was perfused into stomach.Expression of Bcl-2,PCNA in histological specimen of mammary glands in each group in rats was detected with IHC.[Result] Tongru Sanpi decoction could regulate the expression level of Bcl-2,PCNA of mammary glands in experimental rats.[Conclusions] Tongru Sanpi decoction can probably inhibit the hyperplasia of mammary glands by inhibiting cell proliferation and inducing cell apoptosis.