[Study on stability of baicalin and baicalein in Rat plasma by HPLC-ECD].

Objective: To compare the stability of baicalin and baicalein in buffered aqueous solutions and mouse plasma in order to discuss the degradation mechanism and the method of disposing samples.Method: HPLC-ECD was used to determine baicalin and baicalein in different conditions,and the disposing samples methods were selected to stabilize them.Result: Baicalin and baicalein in the buffered aqueous solution at pH 7.4 was more stable than that in the mouse plasma.After adding Vit C in the buffered aqueous solution,the stability of baicalin and baicalein could be improved obviously and their remained percents were 102% and 100%.The stability of baicalin and baicalein could be enhanced distinctly in the mouse plasma only when adding Vit C and 1 mol·L-1 HCl,and furthermore their remained percentage was up to 98% and 103%.Conclusion: The stability of baicalin and baicalein is related to the oxidation and enzyme degradation and it can be improved by adding Vit C and 1 mol·L-1 HCl in the solution.