Antibody Humanization: Intra and Inter VH-VL Non-bond Energy Sort Best Humanized Antibody

Antibody humanization of non-human derived antibody reduces immunogenicity of antibody drug. Computer aided antibody humanization has became an efficient and rapid routine process. Our computational humanization pipeline includes CDR grafting onto antibody crystal structure and humanization of CDR grafted antibody structure. Then, intra and inter VH-VL non-bond energy is calculated and sorted for selection best humanized antibody. Compare to experimental dataset, result indicate that intra and inter VH-VL non-bond energy could rank humanized antibody.