Packing density, permeability, and separation efficiency of packed microchips at different particle-aspect ratios.

Abstract HPLC microchips are investigated experimentally with respect to packing density, pressure drop–flow rate relation, hydraulic permeability, and separation efficiency. The prototype microchips provide minimal dead volume, on-chip UV detection, and a 75 mm long separation channel with a ca. 50 μm × 75 μm trapezoidal cross-section. A custom-built stainless-steel holder allowed to adopt optimized packing conditions. Separation channels were slurry-packed with 3, 5, and 10 μm-sized spherical, porous C8-silica particles. Differences in interparticle porosity, permeability, and plate height data are analyzed and consistently explained by different microchannel-to-particle size (particle-aspect) ratios and particle size distributions.