The cellular thermal shift assay for evaluating drug target interactions in cells

Rozbeh Jafari,Helena Almqvist,Hanna Axelsson,Marina Ignatushchenko,Thomas Lundbäck,Paer Nordlund,Daniel Martinez Molina

The cellular thermal shift assay for evaluating drug target interactions in cells

2014

Rozbeh Jafari
Helena Almqvist
Hanna Axelsson
Marina Ignatushchenko
Thomas Lundbäck
Paer Nordlund
Daniel Martinez Molina

Interaction between a drug and its protein target results in a shift in thermal stability of that protein. The cellular thermal shift assay–CETSA–exploits this to characterize intracellular drug-protein engagement.

Keywords:

Cell biology
Drug
Thermal shift assay
Protein target
Thermal stability
Intracellular
Molecular biology
Biology
drug target
Plasma protein binding
proteins metabolism

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