Extracellular Synthesis of SilverNanoparticles Using Actinomycetes andtheir Antibacterial Activity

In the present study, isolate and identify the pigment producing actinomycetes, to identify the bioactive compounds and biologically synthesis silver nanoparticles against pathogenic bacteria. A total of seven actinomycetes were isolated from soil samples and tested against pathogenic bacteria. Out of seven isolates, three isolates [A1, A3 and A7] showed better result against pathogenic bacteria by primary screening. Out of three isolates, A1 and A7 were subjected to secondary screening, identification, growth study (temperature and pH), mass multiplication and synthesis of silver nanoparticles methods. The potential isolates was identified by morphological and biochemical tests (Streptomyces sp.). The optimum temperature as room temperature 28oC and favorable pH was 7. The cell free extract of potential isolate was assessed for the synthesis of silver nanoparticles. The biologically synthesized silver nanoparticles showed major peak at 420 nm. Synthesized nanoparticles also exhibited antibacterial activity against bacterial pathogens