Measurements in dynamics of cells membrane modifications induced by free radicals of oxygen attacks by spectropolarimetry and biochemical techniques

Early detection of malignant transformation is a goal of modern medicine and therefore there is an impressive number of approaches from the scientific field that tries to identify early changes preceding malignant transformation in order to establish a correct diagnosis. This paper aims is to combine the optical and biochemical techniques for identifying the changes in membrane dynamics of growth and development of experimental solid tumours. The experimental Walker 256 carcinoma graft in Wistar rats has been used, followed from day 7 up to day 24 from inoculation of tumour cells. Optical techniques were used in fractalometry laser polarization in the preclinical diagnosis of pathological changes and degenerative-dystrophy of experimental tissue, and in terms of biochemical tumour tissue was determined the reaction of lipid peroxidation monitored by the malondialdehyde (MDA), the end product assays of reaction. In addition the total antioxidants as a response of the endogenous defences systems were followed, too. The results indicate a rising profile of the processes investigated by the 14th day after tumour graft, following a decrease due to the lack of substrate enzymatic reactions, specifically the double links of polyunsaturated fatty acids in the membranes change during tumour development.