Polysaccharide components from the scape of Musa paradisiaca: main structural features of water-soluble polysaccharide component

Polysaccharide components present in the pseudo-stem (scape) of M. paradisiaca were purified from acetone powder of the scape by delignification followed by extraction with aqueous solvents into water soluble polysaccharide (WSP), EDTA-soluble polysaccharide (EDTA-SP), alkali-soluble polysaccharide (ASP) and alkali-insoluble polysaccharide (AISP) fractions. Sugar compositional analysis showed that WSP and EDTA-SP contained only D-Glc whereas ASP contained D-Glc, L-Ara and D-Xyl in ∼ 1:1:10 ratio, respectively, and AISP' contained D-Glc, L-Ara and D-Xyl in ∼ 10:1:2 ratio, respectively. WSP was further purified by complexation with iso-amylalcohol and characterized by specific rotation, IR spectroscopy, Iodine affinity, ferricyanide number, blue value, hydrolysis with α-amylase and glucoamylase, and methylation linkage analysis, and shown to be a amylopectin type α-D-glucan.