Fluorescence Resonance Energy Transfer between CdTe QDs and Rhodamine B in Gelatin Nanoparticles via a Two-Step Desolvation Way

A facile method was developed for preparation of fluorescence resonance energy transfer between CdTe QDs (donor) and rhodamine B (acceptor) in gelatin nanoparticles. The fluorescent gelatin nanoparticles embedded CdTe QDs and rhodamine B were prepared by a two-step desolvation way and characterized by UV–Vis spectrometer, spectrofluorometer, transmission electron microscope and fluorescent microscope. The prepared method allowed for short acceptor-donor separation and thus for high energy transfer efficiencies. Energy transfer efficiency (E) and distance (r) between CdTe QDs and rhodamine B were obtained to be 26.5% and 6.84 nm, respectively. The fluorescence resonance energy transfer system was proved to be formed successfully, indicating that the fluorescent gelatin nanoparticles were expected to be promising candidates for biological imaging studies.