Human papilloma virus 16 type DNA vaccine and gene adjuvant and its application

The invention discloses a human warts virus 16 type (HPV16)DNA vaccine antigen gene, fuse antigen gene, eucaryon expressing plasmid and construction eucaryon expressing plasmid of IL-15 ripe peptide cDNA with human IL-2 signal peptide on the vaccine adjuvant gene N end, which is characterized by the following: possessing stronger immunogen property; generating more CD8 T cells with E7 specificity; preventing formation of transferring tumor with avoidance immune; co-immunizing with IL-15 gene adjuvant; further-improving antineoplastic activity of the vaccine especially immunization therapy activity of tumor; removing tumor cell in mouse body completely with jointing IL-15 gene adjuvant. This DNA vaccine and gene adjuvant can be used to prevent and cure carcinomatous conversion and immunization therapy of carcinoma.