Broadband extraction of blood for mass spectrometric protein profiles

The invention relates to the preparation of samples for mass spectrometric determination of protein profiles from blood, wherein the proteins being measured with reproducible conditions as possible their concentration obtained by a broadband extraction. The protein profiles can be used for example for medical diagnosis, but also to monitor the effectiveness of medications. The invention provides a sample preparation method that carries out the broadband extraction by reversible immobilization on actively binding solid surface after removal of the blood still in the medical laboratory from the fresh whole blood, blood plasma or blood serum and only the extracted substances, preferably in the immobilized state, the mass spectrometric supplies laboratory for measurement of solids profile. Thus, is largely avoided that the substance concentrations change unreproducible by chemical or enzymatic processes in the always reactive blood during alternating long transport times, and it can dispense with the addition of enzyme inhibitors or freezing to minus 80 ° C for transport to the mass spectrometry laboratory become.