Unpowered micro-flow control chip for heavy metal ion on site detection and making and using method thereof

The invention discloses a multi-channel powerless microfluidic chip for field detection of heavy metal ions and a production method and a use method thereof. The microfluidic chip comprises a substrate layer and a channel sheet layer hermetically connected with the substrate layer, wherein the channel sheet layer comprises a single or more channels, and both ends of the channels are connected with sample inlet holes and sample outlet holes respectively; the substrate layer and the channel sheet layer are both made of polydimethylsiloxane materials, and the thickness of the channel sheet layer is between 8 and 20 millimeters. A sample inlet mode of the chip does not need external energy, and can achieve the field use effect only by utilizing the ability of gas storage of polydimethylsiloxane through the vacuum-pumping pretreatment. The microfluidic chip is suitable for the real-time rapid detection of different kinds of heavy metal ions in the field when being combined with different kinds of nanometer gold probes.