PRELIMINARY CYTOTOXIC EVALUATION OF Andrographis paniculata IN BREAST CANCER CELL LINES

Sambiloto ( Andrographis paniculata ) banyak digunakan untuk mengobati berbagai penyakit di Indonesia dan negara-negara Asia lainnya. Dalam studi ini, ekstrak metanol dan etanol s ambiloto yang diperoleh dari B2PTO Tawangmangu telah diuji terhadap s el lin i kanker  payudara T47D dan MCF- 7 dan sel lin i normal  fibroblast HFL-1 menggunakan reaksi enzimatik 3-(4,5-dimethylthiazoyl-2-yl) 2,5 - diphenyltetrazoliumbromide (MTT ). Uji in vitro terhadap sel lin i normal fibroblast HFL-1 menunjukkan bahwa 50 ppm ekstrak metanol s ambiloto tidak menghambat pertumbuhan sel. Tetapi, ekstrak metanol dan etanol nya menghasilkan IC 50 yang relatif rendah pada sel lini kanker payudara, yaitu 111 ppm dan 122 ppm pada sel lin i MCF-7 dan 70 ppm dan 197 ppm pada sel lin i T47D. Selain itu, campuran ekstrak s ambiloto yang mengandung 25% ekstrak Thyponium divaricatum dan Anredera cordifolia memberikan daya hambat pertumbuhan pada sel kanker payudara MCF-7 yang lebih besar, dengan nilai IC 50 masing-masing adalah 68 ppm dan 34 ppm. Kesimpulannya, total ekstrak metanol atau etanol s ambiloto yang diperoleh dari Tawangmangu memiliki potensi sebagai sumber senyawa anti-kanker serta perlu kaji an lebih lanjut . Kata kunci: Ekstrak Andrographis paniculata , MTT , sel lini normal , sel lini kanker , aktivitas anti kanker ABSTRACT Sambiloto ( Andrographis paniculata ) is widely used as medicine to treat various diseases in Indonesia and other Asian countries. In this study, methanolic and ethanolic extracts of sambiloto collected from B2PTO Tawangmangu have been tested againts breast cancer cell lines of T47D and MCF-7 and normal fibroblast cell line of HFL-1 using enzymatic reaction of 3-(4,5-dimethylthiazoyl-2-yl) 2,5-diphenyltetrazoliumbromide (MTT). In vitro assay performed on normal fibroblast of HFL-1 cell line showed that 50 ppm of methanolic extract of sambiloto did not inhibit cell growth. However, methanolic and ethanolic extracts of sambiloto gave relatively low of IC 50 on breast cancer cell lines which were 111 ppm and 122 ppm on the MCF-7 cell lines and 70 ppm and 197 ppm on the T47D cell lines, respectively. In addition, the mixture of sambiloto extract containing 25% of Thyponium divaricatum and Anredera cordifolia extracts confered greater growth inhibition on breast cancer cell line of MCF-7, where IC 50 values were 68 ppm and 34 ppm, respectively. In conclusion, the total methanolic or ethanolic extract of sambiloto collected from Tawangmangu has potency as a source of anti-cancer compounds and needs further study. Key words: Andrographis paniculata  extract, MTT, normal cell line, cancer cell lines, anti-cancer activity