Construction of a Cellular Model for Drug Screening of Duchenne Muscular Dystrophy

To establish a convenient method for observing the expression of utrophin and a cellular model for drug screening of duchenne muscular dystrophy.Three fragments of utrophin gene at 18 exon and 19 exon and the sequence surround them by PCR.TETC gene was inserted in 3' of fragment I.The target gene was constructed with pGPKneobpA-LoxP-A and fragment Ⅰ,Ⅱ,Ⅱ,then transformed into C2C12 cells by electroporation.To identify homologous recombinants,transfected C2C12 cells were screened by G418,FlAsH staining and PCR detection.The target gene sequence was correct by sequencing,and two homologous recombinated C2C12 cells were got.A cellular model for detecting utrophin expression and a platform for drug screening of duchenne muscular dystrophy were constructed.