Stability Indicating Analytical Method Development and Validation of Ciprofloxacin By RP-HPLC with Fluorescence Detector

Ciprofloxacin is an anti-microbial agent, member of the quinolone class compound with activity was found against gram-positive and negative bacteria as well as wide clinical applicability. A simple and sensitive nanomolar concentration for the quantification of ciprofloxacin (QoC) using reverse phase high-performance liquid chromatography (HPLC) method with fluorescence detection. The method was performed using a C18 column at 40⁰C temperature and 0.1% orthophosphoric acid and methanol with a ratio of 70:30 (v/v). The mobile phase was used for the separation of ciprofloxacin. The wavelength of measurement was set at excitation 278 nm and emission 455nm. The method was developed in a 50 to 250 ng/mL concentration with a correlation coefficient of 0.9918. The limit of detection (LoD) and limit of quantification (LoQ) for ciprofloxacin was found to be 0.3215 ng/mL. and 0.9743 ng/mL respectively. The method was sensitive, stability-indicating, and rapid for nanomolar determination of ciprofloxacin in the pure and pharmaceutical dosage form. The analytical procedure was specific and validated as per the ICH guideline (Q2R1).