Multidimensional fl uorescence microscopy in live cell imaging - A mini review Bildgebende multidimensionale Fluoreszenzmikroskopie an lebenden Zellen

Fluorescence microscopy methods are described with high spatial, spectral, and temporal resolution. In addition to threedimensional (3D) microscopy, based on confocal, structured, or single-plane illumination, spectral imaging and fl uorescence lifetime imaging microscopy are used to probe the conformation of flmolecules as well as their interaction with the microenvironment. In addition to single cells or cell monolayers, 3D cell cultures are used increasingly, as they are more representative for tissue morphology and function. All methods are discussed in the context of controlled light exposure, which is regarded as a key parameter to maintain cell viability. The applications presented in this mini review include autofl uorescence measurements of glioblastoma cells as well as various fl uorescent markers or fl proteins.