Development of a disposable and low-cost electrochemical sensor for dopamine detection based on poly(pyrrole-3-carboxylic acid)-modified electrochemically over-oxidized pencil graphite electrode

Abstract In this study, preparation of a single-use electrochemical sensor for the selective and sensitive determination of dopamine (DOP) was investigated by electrochemical polymerization of pyrrole-3-carboxylic acid on electrochemically over-oxidized pencil graphite electrode (p(P3CA)/EOPGE). Cyclic voltammetry measurements of Fe(CN) 6 4−/3− indicated that the electrochemically over-oxidized PGE (EOPGE) showed superior electron transfer characteristics according to bare PGE. The ionized carboxyl groups found in the structure of poly(pyrrole-3-carboxylic acid) (p(P3CA)) showed high affinity towards positively charged DOP. The combination of the advantages of EOPGE and p(P3CA) in p(P3CA)/EOPGE led to a synergistic effect on the electrochemical oxidation of DOP. The effects of experimental variables on the voltammetric performance of the p(P3CA)/EOPGE were examined by preparing the electrodes at different conditions. The p(P3CA)/EOPGE showed high selectivity towards DOP by discriminating its oxidation potential from the common interfering substances such as ascorbic and uric acids. The p(P3CA)/EOPGE showed linear responses in the electrochemical oxidation of DOP between the concentration values of 0.025 µM and 7.5 µM. Detection limit was determined as 0.0025 µM according to signal to noise ratio (S/N: 3). Analytical application of p(P3CA)/EOPGE was successfully tested in the determination of DOP in blood serum and pharmaceutical samples.