Synthesis and Application of Sulfated β-Cyclodextrin Polymer and Its Application in Chiral Separation by Capillary Electrophoresis

The β-cyclodextrin polymer obtained by the reaction of β-cyclodextrin with epicholrohydrin was sulfated with sulfur trioxide-pyridine complex to obtain a sulfated β-cyclodextrin polymer.The sulfated β-cyclodextrin polymer was characterized with IR and 1H NMR.As chiral additive,the sulfated β-cyclodextrin polymer was used in capillary zone electrophoresis for enantioseparation.The effects of pH value,concentration of additive,running voltage and organic solvent on the enantioseparation were investigated.The results of 1H NMR indicated that the substitution degree of the polymer was not uniform.The results of capillary electrophoresis showed that the sulfated β-cyclodextrin polymer had good chiral separation ability although it was not uniform substituted.Five racemic chiral drugs,terbutaline,anisodamine,chlorphenamine,omeprazole,and ofloxacin,were successfully separated into their isomers.Under the conditions of 5 g/L sulfated β-cyclodextrin polymer and 15 kV running voltage,the resolutions were more than 3.0 for terbutaline and chlorphenamine,and the resolutions for anisodamine enantiomers were both above 2.0.