SECOND DERIVATIVE SPECTROPHOTOMETRIC METHOD FOR THE ESTIMATION OF METFORMIN HYDROCHLORIDE IN BULK AND IN TABLET DOSAGE FORM Research Article

A simple, economic, sensitive, precise and accurate second derivative spectrophotometric method has been developed for determination of Metformin hydrochloride in bulk and in tablet dosage form. The quantitative determination of the drug was carried out using the second derivative values measured at 233.8 nm. Calibration graph constructed at 233.8 nm was linear in concentration range of 4-20 µg/ml with correlation coefficient 0.9979. The method was validated as per ICH guidelines and can be used for determination of Metformin hydrochloride in tablet dosage form.