Validation of nonlinear interferometric vibrational imaging as a molecular OCT technique by the use of Raman microscopy
2009
We validate a molecular imaging technique called Nonlinear Interferometric Vibrational Imaging (NIVI)
by comparing vibrational spectra with those acquired from Raman microscopy. This broadband coherent
anti-Stokes Raman scattering (CARS) technique uses heterodyne detection and OCT acquisition and design
principles to interfere a CARS signal generated by a sample with a local oscillator signal generated
separately by a four-wave mixing process. These are mixed and demodulated by spectral interferometry. Its
confocal configuration allows the acquisition of 3D images based on endogenous molecular signatures.
Images from both phantom and mammary tissues have been acquired by this instrument and its spectrum is
compared with its spontaneous Raman signatures.
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