Characterization of Nano Features in Biopolymers using Small‐angle X‐ray Scattering, Electron Microscopy and Modeling

The sections in this article are Introduction Small-angle X-ray Scattering Scattering Technique Scattering Phenomenon Scattering Curve and Pair Distance Distribution Function Determination of Scattering Parameters Experimental Setup Interpretation of Data Direct Methods Indirect Methods Electron Microscopy Image Formation Interference of Electrons with Matter Contrast Transfer Function Sample Preparation Vitrification of Biological Specimens Two-dimensional Merging of Electron Microscopic Data Cross Correlation Function Identification of the Different Views Merging of EM-data in Three Dimensions Sinogram Correlation Reconstruction of the Three-dimensional Model Merging of Methods Comparison of EM and SAXS Data SAXS Modeling Approaches using EM Information