Characteristics of hydrolysis of lipopolysaccharide and Boivin antigen isolated from Salmonella typhimurium

: The work deals with the isolation of serologically active polysaccharide from S. typhimurium 415. Particular attention is paid to different conditions for the hydrolysis of lipopolysaccharide (LPS) and Boivin's antigen, permitting the authors to obtain polysaccharides with different activity of the determinant groups. The action of 1% acetic acid on LPS, lasting for several hours, did not result in the hydrolysis of the antigen, while its hydrolysis in 1.5% acetic acid led to the decomposition of the greater part of the determinant groups. Polysaccharide isolated from Boivin's antigen after hydrolysis in 1% acetic acid retained the specific activity of all antigenic determinants of the natural biopolymer.