In Vivo Antioxidant Activity of Black Soybean Peptide in Aging Mice Caused by D-Galactose

Black soybean peptides (BSPs) with potent antioxidant activity were purified from black soybean protein hydrolysates (BSH) by using the ultrafiltration (UF) and macroporous adsorption resin (MAR), and in vivo antioxidant activity of the fraction BSP-DA-c was evaluated in aging mice induced by D-galactose. The results showed that orally administration of BSP-DA-c fraction at the dose of 500 and 1000 mg/kg per day could remarkably increase (P<0.05) the activity of SOD and GSH-Px in liver and the activity of GSH-Px in serum, the contents of MDA in serum and liver were reduced significantly (P<0.05), inferred that BSP-DA-c had strong antioxidant activity in mice.