Karyotyping of Fusarium oxysporum by Pulsed-Field Gel Electrophoresis and the Germ Tube Burst Method.

In fungi, karyotyping is fundamental to understanding their genome organization. It is also essential to study various genome- or chromosome-related topics such as karyotype polymorphisms and supernumerary or pathogenicity chromosomes. Here, we describe the protocols of pulsed-field gel electrophoresis and the germ tube burst method for molecular and cytological karyotyping of Fusarium oxysporum. The combined use of the two methods is valuable for determining definitive and comprehensive karyotypes of these fungi.