Role of hypothermia induced by tumor necrosis factor on apoptosis and function of inflammatory neutrophils in mice

2000 
Changes in body temperature and cell infiltration, mediated by cytokines including tumor necrosis factor-α (TNF-α), occur during inflammation, but a role of body temperature on inflammatory responses remains obscure. Intraperitoneal injection of 10% casein to mice resulted in transient hypothermia followed by neutrophil accumulation in peritoneal cavities. Peritoneal TNF-α was rapidly raised, and pretreatment of mice with an anti-TNF-α antibody promoted temperature restoration and partially inhibited neutrophil accumulation. To investigate direct effects of body temperature on neutrophils, peritoneal or peripheral blood neutrophils were cultured at 35°C or 37°C with or without recombinant murine TNF-α (100 ng/ml) or a protein synthesis inhibitor cycloheximide (1 μg/ml). Significant inhibition of spontaneous and TNF-α-induced apoptosis was obtained at 35°C compared with 37°C, an effect that was not altered by the addition of cycloheximide. Moreover, phagocytic ability of peritoneal neutrophils was significantly enhanced by incubating them at the lower temperature. These results indicate that mild hypothermia induced by endogenous TNF-α has enhancing roles on neutrophil survival and function during peritoneal inflammation.
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