Tissue Culture and Rapid Propagation of Fine Clone for Cunninghamia lanceolata (Chenshan-red-fir)

The tissue culture and rapid propagation system of Cunninghamia lanceolata (Chenshan-red-fir)were established, based of basal branches of fine clones for twenty materials, which are above 70% on red-heart proportion. The result showed: the culture medium of induced budding was optimum based on MS+BA2.0mg/L+NAA0.5; the culture medium of multiplication was optimum based on 1/2MS+BA1.5+NAA0.5+right amount organic matter, in which the leaves grow green and fine, and the multiplication averagely reached about 4.5 times; the rooted culture medium is optimum based on 1/2MS+NAA0.2+IBA0.5, and the rooting ratio can be up to 89.3%.