Biosynthesis, optimization, purification and characterization of gold nanoparticles

-4 mM HAuCl4 solution as compared to other dilutions. The pH 7.0 was found to be optimum for the biosynthesis of gold nanoparticles. The TEM study revealS that there was evidence of gold nanoparticles synthesized by S. hygroscopicus. The results demonstrate that spherical gold nanoparticles in the range of 10 to 20 nm were observed at pH value of 7.0. The actinomycetal biomass and various concentration of aqueous HAuCl4 solution were incubated, it was found that 10 -4 concentration shows excellent colour of the actinomycetal biomass.