Effect of different media, temperature and pH on radial mycelial growth of Lentinula edodes strain Le-17-04

Lentinula edodes (Berk.) Pegler is one of the lignicolous mushrooms that produces brown sporocarps. Presently standardized cultural conditions for shiitake mushroom is unavailable. So the present study has been conducted for better understanding of cultural conditions for shiitake mushroom. Under in-vitro study the vegetative growth of Le-17-04 was also evaluated on culture media, pH and temperature. It was found that Lentinula edodes Le-17-04 was well grew best on PDA medium at 24oC and at both pH 5.0 and pH 6.0 with maximum 90 mm, 83.33 mm and 90 mm radial growth at 15, 12 and 12 days after inoculation, respectively.